The identification of variety authenticity is a key link in crop variety identification and intellectual property protection, and it is necessary to ensure the specificity, consistency and stability of varieties through DUS testing to prevent "false seeds" from entering the market and harming breeding innovation. Current mainstream detection methods include SSR and SNP molecular marker technology. For example, corn varieties can be identified by 40 pairs of core primers, but their labeling density is low and the method is single, making it difficult to cope with the surging detection needs. As a third-generation marker technology, SNP is more suitable for high-throughput automated detection and significantly improves identification efficiency by virtue of its high genome density, genetic stability, and simple data statistics. In addition, with the relaxation of transgenic policies, the demand for pure heterozygous detection in transgenic breeding has increased, and this process needs to be supported by high-precision instruments and strict laboratory systems, which has become an important direction for the development of breeding technology. The iteration of molecular detection technology is driving variety identification towards higher accuracy and efficiency.