Variety Authenticity Identification - SSR - RocGene (Beijing) Technology Co., Ltd.

Application Requirements

SSR (Simple Sequence Repeat) is a molecular labeling technology based on microsatellite DNA. Its core principle is to amplify short tandem repeats (usually 2-6 base repeat units) at specific locations in the genome through PCR, and identify polymorphisms caused by differences in the number of repetitions between different cultivars. SSR markers have the characteristics of flanking sequence conservation, and after amplifying the target region by designing specific primers, the length difference of the amplified fragments is analyzed by electrophoresis or capillary electrophoresis to construct a DNA fingerprint.

Solutions

Nucleic Acid Amplification Solution - ArchiSuit + RocStation + ArchiCycler96

Sample Processing: High-purity nucleic acid is extracted from samples (e.g., seeds) using 16/32/96 Pro throughput fully automated nucleic acid extractor, improving the sensitivity and reliability of subsequent detection.
System Preparation: The automated PCR liquid handling system (RocStation) enables plate-based PCR reaction system preparation, or Rocgene lyophilized ready-to-use reagents can be adopted; purified nucleic acid after extraction can also be transferred in plates, significantly improving workflow efficiency.
Amplification Results: Amplification is performed using gradient PCR instrument (ArchiCycler 96); PCR products are subjected to electrophoresis to verify variety authenticity. Reaction conditions can be optimized according to the characteristics of variety-specific detection primers, ensuring high specificity of results.

Application Scenarios

Research Institutes

Used in breeding research to assist in the creation of new crop varieties.

Testing Service Companies

Provide variety identification services for clients and resolve disputes over existing varieties.

Regulatory Authorities

Resolve disputes over existing varieties to maintain fairness in the seed industry market; supervise seed quality, combat counterfeit and shoddy products, protect farmers' rights and interests, and ensure food security.

Detection Workflow

Nucleic Acid Amplification Solution - ArchiPure 96 Pro + RocStation + ArchiCycler96

Our Advantages

High-Efficiency Detection

The entire product series can be controlled by a single terminal, enabling full-process automation and high-throughput sample testing.

Superior Instrument Performance

Ultra-high temperature control precision effectively ensures amplification efficiency, and faster temperature ramping rate reduces detection time.

Flexible Solution Design

Customizable complete laboratory design and detection solutions; one-stop configuration of related instruments and testing services is available.

Comprehensive After-Sales Service

Lifetime free software upgrades; professional software team provides customized software development according to specific requirements.

Success Cases

Scenario: An SSR project testing service company in Liaoning Province

Requirements: Due to the large number of samples to be tested, a large number of gradient PCR instruments were needed, with emphasis on shortening detection time to improve testing turnover rate and control hardware and personnel investment costs.

Solution:

The temperature ramping rate of ArchiCycler series gradient PCR instrument is 12℃/second, which significantly reduces detection time compared with conventional instruments with a ramping rate of about 5℃/second. The overall detection time was shortened from 1 hour to 45 minutes.

Results:

By specifically adapting to the existing primers in the laboratory, ArchiCycler greatly improved detection efficiency and reduced testing costs. Multiple sets of our equipment were subsequently introduced in batches for testing.

Laboratory Installation

Detection Results