Rocgene pathogenic microorganism and biopharmaceutical extraction kit adopts high-efficiency magnetic nanoparticles and an optimized buffer system, which can quickly capture target DNA or RNA from a variety of clinical samples such as whole blood, plasma, serum, throat swab, alveolar lavage, secretion, cells, and cultures, and is suitable for automated workstations or manual operation. Nucleic acids are efficiently released through lysate, and after the specific adsorption and binding of magnetic beads, high-purity and high-integrity nucleic acid products are finally obtained through multi-step impurity removal washing, which is compatible with downstream PCR, qPCR, next-generation sequencing and other molecular detections. The kit does not require toxic reagents such as chloroform throughout the whole process, has a short operation time, flexible throughput (1-96 samples), and an extraction efficiency of > 90%.