Pathogen load detection - RocGene (Beijing) Technology Co., Ltd.

Application Requirements

In clinical diagnosis, the diagnosis of many patients not only needs to confirm whether they are infected, but also needs to grasp the infection load of pathogens (such as hepatitis B, AIDS) in time, and the results of pathogen load detection directly affect the formulation of clinical treatment plans. Changes in pathogen load after treatment are also important indicators for evaluating treatment efficacy. Therefore, healthcare facilities and public health departments urgently need a stable, easy and accurate pathogen load detection protocol.

Solutions

Open Workstation Solution - Atlas120

Sample Processing: Supports fully automated nucleic acid extraction for up to 12 samples simultaneously.
Assay Preparation: Open detection reagents with robotic arm for automated reaction preparation, full liquid sealing to avoid contamination.
Detection Results: Standard real-time fluorescent quantitative PCR reactions supporting multiple reaction types including positive/negative identification, absolute quantification, and relative quantification; enables on-demand testing.

Standard Detection Solution - ArchiPure 32/96 + Archimed X

Sample Processing: High-purity nucleic acid is extracted from samples (e.g., blood) using 32/96-throughput fully automated nucleic acid extractor, improving the sensitivity and reliability of subsequent detection.
Assay Preparation: Manual preparation of detection system.
Detection Results: Standard 96-throughput real-time fluorescent quantitative PCR instrument combined with multiplex PCR technology, supporting multiple reaction types including positive/negative identification, absolute quantification, and relative quantification; meets daily detection needs of standard-throughput experiments.

Application Scenarios

Infectious Disease Department, Hepatology Outpatient & Inpatient Wards of Hospitals

Used for disease assessment, treatment monitoring and follow-up management of patients with chronic hepatitis B and AIDS. Physicians adjust the type, dosage and treatment cycle of antiviral drugs based on changes in viral load.

Physical Examination Centers

Screening for hepatitis B and HIV infections in general population and high-risk groups to detect potential infections in a timely manner and provide basis for subsequent diagnosis and treatment.

Blood Centers

Nucleic acid testing for hepatitis B virus (HBV) and HIV in blood donors to reduce the risk of transfusion-transmitted diseases and ensure the safety of blood products.

Hospital Research

Used for basic and clinical research related to hepatitis B virus, exploring the pathogenesis of hepatitis B, and evaluating new therapeutic methods and drug efficacy.

Detection Workflow

Open Workstation Solution - Atlas 120

Standard Detection Solution - ArchiPure32/96 + Archimed X

Our Advantages

High Sensitivity

Capable of detecting extremely low levels of pathogens; especially when paired with high-sensitivity detection kits, it can detect viral loads as low as 10 - 20 IU/mL, helping to identify occult infections and monitor patients with low viremia.

Strong Specificity

Rapid temperature ramping avoids the impact on enzyme performance during temperature changes, shortening detection time while ensuring detection stability even at extremely low viral loads, resulting in highly specific results.

Accurate Quantification

Quantification method based on standard curve can accurately determine the copy number or IU/mL of viral nucleic acid, providing reliable quantitative indicators for clinical diagnosis and treatment.

Rapid and Efficient

The entire detection process is usually completed within 1-1.5 hours, reducing detection time by more than 15% compared with other nucleic acid testing instruments, enabling faster clinical decision-making.

Dynamic Monitoring

Can be used for dynamic monitoring during anti-infective treatment, reflecting changes in viral load in a timely manner, helping physicians evaluate treatment efficacy, adjust treatment plans, and predict disease progression and prognosis.

Success Cases

Scenario: As a regional medical center, the First People's Hospital of XX City receives over 3,000 patients with chronic hepatitis B annually and needs to complete approximately 1,500 hepatitis B virus (HBV) quantitative testing samples per month. Testing scenarios include initial screening of outpatients in the Infectious Disease Department, antiviral treatment monitoring of inpatients, screening of high-risk groups in the Physical Examination Center, and nucleic acid testing of blood donors in the blood bank. In practical work, traditional testing equipment has long detection time, requires regular light source replacement, and has high calibration and maintenance costs, often resulting in patients waiting more than 48 hours for test reports, and it is difficult to meet emergency testing needs under public health emergencies.

Requirements: Need to meet the testing throughput of 50 - 100 samples per day, control single-batch detection time within 1.5 hours to shorten patient waiting time and improve clinical diagnosis and treatment efficiency. Mean Time Between Failures (MTBF) of 330 days/year, with low maintenance cost ≤ 3,000 CNY/year.

Solution:

Through cooperation with a high-sensitivity HBV detection kit manufacturer and passing the reagent compatibility test, the original outdated equipment was successfully replaced. Archimed X and ArchiPure32 were installed in the clinical laboratory of the hospital through the reagent manufacturer.

Results:

Since the introduction of Archimed quantitative PCR instrument, the HBV testing efficiency of the First People's Hospital of XX City has been significantly improved: the average patient waiting time for test reports has been reduced from 48 hours to 12 hours, and outpatient satisfaction has increased to 95%. The high sensitivity of the instrument has increased the detection rate of patients with low viral load by 30%, providing key data support for clinical precision treatment. The light source of Archimed requires no maintenance for life, greatly reducing the after-sales cost of the hospital, and the amplification time for HBV testing has been reduced from 102 minutes to 63 minutes.

On-site Usage Photos

Amplification Results of a HBV Detection Kit